Micrografting technique in cinchona (Cinchona ledgeriana Moens) plants propagation
In Indonesia has been found 11 cinchona species but only two species recognized as an economically important Cinchona succirubra known as resistant plant to root disease while Cinchona ledgeriana quinine high content. To put together those two characters from mostly through grafting it took twelve months. Micrografting is one of the propagation techniques through tissue culture. Micrografting has already been used in wide spread for plants propagation either only for research or as mass product. Hopefully, the use of micrografting to obtain cinchona propagation would be short more or less 10 months and provide plants as mass production economically. The result showed that micro grafting at V shape could be used as the propagation method on cinchona species besides bud multiplication through tissue culture. MS medium is the best medium for cinchona micrografting. If there is no IBA on rooting media, there is no root formation from the plants. The increasing of IBA from 1 up to 4 mg/l in media, will increase also the number of rooting plants.different plant material as scion and stock and different concentrarions (0,1,2,3,4 mg/l) of IBA gives the significantly different respons on rooting formation. The increasing of IBA on media will increase the number of rooting plants also. The highest rooting formation are found on medium with 3 danÂ 4 mg/LÂ IBA. The combination between C. ledgeriana-C. succirubra, C. ledgeriana-C.ledgeriana, C. succrirubra-C.succirubra gives significantly difference on the growth dan development of root. The use of C. ledgeriana as a root stock doesnâ€™t showed good rooting formation. The combination between CSA/QRC205 gives the highest number of leaves result compare wth others combination on as much as 9,8. The first stage of acclimatization on culture room with temperature 25-270C and followed by acclimatization on plastics house covered with paranet 70%. In general in vitro planlet cinchona from micrografting growing well. By the end of observation (8 weeks) growing percentage reach 90%.Â Â
Copyright (c) 2016 Jurnal Penelitian Teh dan Kina
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
a. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
b. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
c. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeÂ The Effect of Open Access).