In-vitro experiment of nitrogen fixing capacity of indigenous and exogeneous Azotobacter sp at Andisol tea planting areaâ€™s
An in-vitro experiment to evaluate total nitrogen content of soil of tea plantation area included with indigenous and exogenous AzotoÂbacter sp have been conducted in the Biology and Soil Biotechnology Laboratory, PadjaÂdjaran University. A completely randomized design with 11 treatments and three repliÂcations was used. The treatments were: A (neÂgative control); B (positive control); C (A. Kedelai II); D (A. vinelandii); E (A. N.D.9.3); F (A. Padi); G (VI-1); H (II-1); I (V-2); J (I-1); and K (III-2). Size of microbe inoculated popuÂlation for each treatment was 1,2 x 1011 CFU/ml. One percent dose was used for steÂrilized soil originated from the tea plantation area. After five days of incubation period reÂvealed that II-1 Azotobacter sp produced very significantly different in total nitrogen proÂduction than the other Azotobacter sp, i.e. 0,8532%. Orthogonal comparisons indicated that indigenous Azotobacter sp had the capacity of nitrogen fixation 7,67% higher than that the capacity of nitrogen fixation of exogenous Azotobacter sp for growth on Andisol soil of tea plantation area with pH of 4,5-5,6. Although, Azotobacter sp is very rarely found in soil with low pH (high acidity), this phenomena indiÂcating that an active biofertilizer of indigenous Azotobacter sp is present in tea plantation area.
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